Pilot Study of "Bottarga" Supplementation: A Little-known, Sustainable "Blue" Food

Purpose

This pilot study aims to explore the potential benefits of consuming Greek bottarga (grey mullet fish roe) in individuals with at least one metabolic abnormality (low HDL cholesterol, high LDL cholesterol, high triglycerides, obesity, or HbA1c ≥ 5.7%) or a diagnosis of diabetes. Before initiating the crossover randomized controlled trial (RCT), the investigators will conduct a preliminary dose-testing study in five adults with at least one metabolic abnormality. Participants will undergo clinical assessments before and after the dietary intervention to evaluate changes in metabolic health markers. Following this phase, the investigators will proceed with a randomized, controlled crossover trial involving 20 eligible adult participants. This main study phase will compare the metabolic effects of daily bottarga supplementation with those of a calorically matched dairy product over an 8-week intervention period, with a 2-week washout period between interventions. The investigators anticipate that bottarga supplementation will improve lipid profiles, inflammation markers, and insulin resistance, thereby supporting the potential use of sustainable blue foods as part of a healthy diet.

Conditions

  • Prediabetes
  • Diabetes
  • High Cholesterol/Hyperlipidemia
  • Obesity & Overweight
  • Triglycerides
  • HDL Cholesterol

Eligibility

Eligible Ages
Between 18 Years and 60 Years
Eligible Sex
All
Accepts Healthy Volunteers
No

Criteria

- Adults aged 18-60 years

- Residents of Massachusetts

- Presence of at least one metabolic abnormality (low HDL cholesterol, elevated LDL
cholesterol, elevated triglycerides, obesity, or HbA1c ≥ 5.7%) or a diagnosis of
diabetes

- Not pregnant

- Willing to consume a nutritional supplement and comply with study procedures

Exclusion Criteria:

- Use of any medications for diabetes (except metformin), dyslipidemia (except
statins), or immunosuppression

- Current use of any supplements containing n-3 fatty acids

- Current use of illicit drugs (other than marijuana)

- Use of hormone therapy (except oral contraceptives)

- Known allergies to fish, seafood, or any fish-derived products, including bottarga

- Pregnancy

- Clinical evidence or history of hepatic or renal insufficiency

- Immunodeficiency conditions

- History of non-skin cancer

- Participation in other clinical research studies

Study Design

Phase
N/A
Study Type
Interventional
Allocation
Randomized
Intervention Model
Crossover Assignment
Intervention Model Description
A randomized, controlled, open-label, crossover pilot study will investigate the metabolic effects of daily Bottarga supplementation, compared to a calorically matched dairy product, in 20 participants with at least one metabolic abnormality (low HDL cholesterol, high LDL cholesterol, high triglycerides, obesity, or HbA1c ≥ 5.7%) or a diagnosis of diabetes. Each intervention will last 8 weeks, separated by a 2-week washout period.
Primary Purpose
Prevention
Masking
Single (Outcomes Assessor)

Arm Groups

ArmDescriptionAssigned Intervention
Experimental
Bottarga supplementation (intervention group)
Ten participants with at least one metabolic abnormality (low HDL cholesterol, high LDL cholesterol, high triglycerides, obesity, or HbA1c ≥ 5.7%) or a diagnosis of diabetes will be randomly allocated to 8 weeks of daily bottarga supplementation (20 grams/day; participants weighing >100 kg [220 lbs] will consume 40 g/day) as an initial treatment. Baseline assessment measures will be repeated, followed by a 2-week wash-out period. Then, assessment measures will be repeated followed by 8 weeks of daily cream cheese supplementation (28 grams/day; participants weighing >100 kg [220 lbs] will consume 56 g/day), followed by final assessment measures.
  • Other: Bottarga
    Participants will consume prepackaged daily doses of Bottarga, each containing the required amount (20 grams/day; participants weighing >100 kg [220 lbs] will consume 40 g/day) for 8 weeks.
Sham Comparator
Cream cheese supplementation (control)
Ten participants with at least one metabolic abnormality (low HDL cholesterol, high LDL cholesterol, high triglycerides, obesity, or HbA1c ≥ 5.7%) or a diagnosis of diabetes will be randomly allocated to 8 weeks of daily cream cheese supplementation (28 g/day) as the initial intervention. Baseline assessment measures will be repeated, followed by a 2-week wash-out period. Then, assessment measures will be repeated, followed by 8 weeks of daily Bottarga supplementation (20 g/day;participants weighing >100 kg [220 lbs] will consume 40 g/day), followed by final assessment measures.
  • Other: Comparator
    Participants will consume 28g of cream cheese/day (participants weighing >100 kg [220 lbs] will consume 56 g/day) for 8 weeks.

Recruiting Locations

Cambridge Health Alliance
Sommerville, Massachusetts 02145
Contact:
Patricia Lenehan
617-665-1580
elidoriki@challiance.org

More Details

Status
Recruiting
Sponsor
Cambridge Health Alliance

Study Contact

Detailed Description

Rationale/goals: Greek Bottarga (Grey mullet fish roe) is a traditional marine or "blue" food "that is produced in a sustainable manner. Bottarga's composition supports that it has excellent nutritional properties, but to date no human/clinical studies have been published. This pilot study will explore the potential benefits of Bottarga consumption in humans. If the results show potential benefits, this would help promote more sustainable blue foods. Methods: Before initiating the crossover randomized controlled trial (RCT), the investigators will conduct a preliminary dose-testing study in five adults with at least one metabolic abnormality, who will consume 20 g/day of Bottarga. Participants will undergo clinical assessments before and after the dietary intervention to evaluate changes in metabolic health markers. The current randomized, controlled, cross-over pilot study (Open Label, Two-Arms) will investigate the metabolic effects of daily bottarga supplementation (versus a calorically equal dairy product) in 20 participants with at least one metabolic abnormality for 8 weeks with a 2-week "washout" period between food supplement arms. The primary outcome will be determined based on the most clinically important results observed during phase 1. Anticipated Results: The investigators expect Bottarga supplementation to be associated with more beneficial changes on lipid profiles, inflammatory markers and insulin resistance compared to baseline measures and to the calorically equivalent comparator food.