Comparison of Root Canal Desinfection Protocols

Purpose

Evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis

Condition

  • Periapical Periodontitis

Eligibility

Eligible Ages
Between 18 Years and 70 Years
Eligible Sex
All
Accepts Healthy Volunteers
Yes

Inclusion Criteria

  • Adult patients able to give informed consent and willing/able to comply with study procedures and follow-up. - Molar teeth indicated for endodontic treatment. - Teeth with intact pulp-chamber walls (no extensive coronal destruction). - Necrotic pulp confirmed by pulp testing. - Clinical and radiographic evidence of asymptomatic apical periodontitis.

Exclusion Criteria

  • Immature teeth - Teeth with calcified or obliterated canals - Teeth with extensive coronal destruction - Teeth with previous endodontic treatment - Symptomatic apical periodontitis - Teeth with periodontal pockets greater than 4 mm - Patients who have received systemic antibiotics in the last 3 months - Patients with a history of diabetes or immune impairment - Pregnant patients

Study Design

Phase
N/A
Study Type
Interventional
Allocation
Randomized
Intervention Model
Parallel Assignment
Intervention Model Description
This is a parallel-group interventional study in which participants are allocated to different treatment arms according to the intervention received (laser or ultrasound), following standardized clinical protocols.
Primary Purpose
Treatment
Masking
None (Open Label)

Arm Groups

ArmDescriptionAssigned Intervention
Active Comparator
Desinfection device 		Ultrasonic Activation: Piezoelectric ultrasonic unit (Dentsply International) with an Irrisafe tip (Satelec/Acteon).
  • Device: Desinfection device
    Desinfection protocol with (LAI): Er,Cr:YSGG laser (2780 nm) delivered with a radial-firing tip.
Active Comparator
Analytical microbiological method 		qPCR assay: Quantifies total bacterial DNA using 16S rRNA gene amplification.
  • Device: Analytical microbiological methods
    Endocator: Measures ATP levels (Endoscore and RLU) to rapidly assess bacterial load in less than one minute.

Recruiting Locations

Advance Education in Endodontics Program
Buffalo, New York 14214

More Details

Status
Recruiting
Sponsor
State University of New York at Buffalo

Study Contact

Stephen Lyon, DDS
(716) 829-6229
smlyon@buffalo.edu

Detailed Description

This study aims to evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis: Er,Cr:YSGG laser activated irrigation and ultrasonic activation using a piezoelectric ultrasonic device (Dentsply International Inc., York, PA, USA) and Irrisafe tip (Satelec/Acteon, Merignac, France), comparing the detection of bacteria before and after instrumentation. Secondary aims are to assess presence of bacteria before and after chemomechanical preparation using two microbiological methods: - ATP level analysis using the Endocator device - Quantitative PCR (qPCR) for total bacterial quantification.