Purpose

The present study will characterize the ability of pregnenolone to reverse the acute intoxication and associated symptoms of cannabis. Healthy adults with a history of cannabis use will be recruited to participate in a placebo-controlled, within-subject crossover study at Johns Hopkins Behavioral Pharmacology Research Unit (BPRU). By clarifying the ability of pregnenolone to reverse cannabis intoxication symptoms, this study will pave the way for larger clinical studies that provide a foundation for the development of future CB1-receptor NAM medications that could be applied in emergency situations and potentially validate pregnenolone as a treatment for cannabis intoxication.

Condition

Eligibility

Eligible Ages
Between 18 Years and 65 Years
Eligible Sex
All
Accepts Healthy Volunteers
Yes

Inclusion Criteria

  • Ages 18-65 - Good general health based on screening procedures (e.g. physical exam, blood testing, psychiatric evaluation) - Systolic blood pressure <140 mm Hg, diastolic blood pressure < 90 mm Hg, and heart rate <110 bpm at screening and at baseline for dosing session - Body mass index (BMI) in the range of 18 to 36 kg/m2 - Cannabis use within the past three years but none in the month prior to the first test session - Negative urine test for illicit substance use and negative breath alcohol test (0% breath alcohol concentration) at screening and before study sessions

Exclusion Criteria

  • Use of psychoactive substances (aside from nicotine, caffeine, and alcohol) in the month prior to study initiation - Current use of over the counter (OTC) drugs, supplements/vitamins, or prescription medications that, in the opinion of the investigator or medical staff, will impact the participant's safety. - Current use of any prescription or non-prescription medications, including herbal medicines and supplements, that are known to interact with cannabis or pregnenolone - Self-report or ECG indicating clinically significant cardiovascular conditions, including coronary artery disease, stroke, angina, uncontrolled hypertension, arrhythmias (e.g. atrial fibrillation), heart valve placement, or TIA in the past year. - History of hormone-sensitive conditions, including but not limited to gynecologic cancers (breast, ovarian, uterine, etc), endometriosis, uterine fibroids, thyroid, pituitary and/or adrenal syndromes, polycystic ovarian syndrome, etc. - Epilepsy or a history of seizures - Any of the following laboratory values during screening or upon admission: - AST > 165 U/L (normal range 19-55) - ALT > 216 U/L (normal range 19-72) - Alkaline phosphatase > 1.5x upper limit of normal (ULN) - Total bilirubin >1.5 ULN - Glomerular filtration rate (EGFR) < 60 ml/min/1.73m2 - Current or past history of meeting DSM-5 criteria for schizophrenia spectrum or other psychotic disorders, or bipolar I or II disorder - Other unstable and/or compromising medical or psychiatric conditions based on clinical interview and/or MINI results that would interfere with participant safety as determined by study physician, including suicidal ideation and/or attempt, psychosis - Previous diagnosis and treatment for Cannabis Use Disorder - Urine drug screen (e.g. Healgen Scientific 14 Panel Rapid Drug Test) indicating the presence of substances including amphetamines, barbiturates, benzodiazepines, cocaine, opioids (including fentanyl), PCP, and THC at screening and prior to study sessions - Breathalyzer screen indicating presence of alcohol at screening and prior to study sessions - Women who are pregnant (as indicated by a positive urine pregnancy test assessed at intake and before each drug session) or nursing - Women who are of childbearing potential and sexually active who are not practicing an effective means of birth control including oral contraceptives, progestin implant, transdermal birth control patch, intrauterine device (IUD) or vaginal ring. Women who report use of condoms or diaphragm must use a "double-barrier" method of contraception (i.e. diaphragm and condoms). - SBP >/= 140, DBP >/= 90, or pulse >/=100 during screening and/or prior to dosing session - Has donated blood within 30 days of the study - Allergy to eggs or other food allergies that would make ingestion of brownie mix unsafe. - Use of concomitant medications, including herbal medicines and botanical supplements, that are strong inhibitors or inducers of CYP3A4 and CYP2C9

Study Design

Phase
Phase 2
Study Type
Interventional
Allocation
Randomized
Intervention Model
Crossover Assignment
Intervention Model Description
Randomized, controlled, within subjects
Primary Purpose
Treatment
Masking
Double (Participant, Outcomes Assessor)
Masking Description
Double blind, double dummy

Arm Groups

ArmDescriptionAssigned Intervention
Placebo Comparator
Placebo Brownie and Capsules
Placebo brownie, 0mg THC; two 0 mg pregnenolone capsules
  • Drug: Placebo
    Placebo capsule, 0mg
  • Drug: Placebo brownie
    Placebo brownie, 0mg THC
Placebo Comparator
Cannabis/THC brownie and Placebo Capsules
25mg Cannabis/THC Brownie; two 0 mg pregnenolone capsules
  • Drug: Cannabis
    Cannabis brownie, 25mg THC
    Other names:
    • delta-9-tetrahydrocannabinol
    • THC
    • delta-9-THC
  • Drug: Placebo
    Placebo capsule, 0mg
Experimental
Cannabis/THC Brownie and Pregnenolone, low dose
25mg Cannabis/THC Brownie; one 250 mg pregnenolone capsule and one 0 mg pregnenolone capsule
  • Drug: Cannabis
    Cannabis brownie, 25mg THC
    Other names:
    • delta-9-tetrahydrocannabinol
    • THC
    • delta-9-THC
  • Drug: Pregnenolone 250 mg
    Pregnenolone, low dose, one 250mg pregnenolone capsule and one 0 mg pregnenolone capsule
    Other names:
    • Pregnenolone
Experimental
Cannabis/THC Brownie and Pregnenolone, high dose
25mg Cannabis/THC Brownie and two 250 mg pregnenolone capsules
  • Drug: Cannabis
    Cannabis brownie, 25mg THC
    Other names:
    • delta-9-tetrahydrocannabinol
    • THC
    • delta-9-THC
  • Drug: Pregnenolone 500 mg
    Pregnenolone, high dose, two 250 mg pregnenolone capsules
    Other names:
    • Pregnenolone

Recruiting Locations

Johns Hopkins University School of Medicine, Behavioral Pharmacology Research Unit
Baltimore, Maryland 21224
Contact:
David Wolinsky, MD
646-572-6959
dwolins2@jhmi.edu

More Details

Status
Recruiting
Sponsor
Johns Hopkins University

Study Contact

David Wolinsky, MD
(646) 572-6959
dwolins2@jhmi.edu

Detailed Description

This human laboratory study will characterize the ability of pregnenolone to reverse the acute cannabis intoxication using measures of subjective drug effects, cardiovascular responses, and cognitive performance. Participants (n=16) will complete four double-blind, randomized, outpatient sessions. In each session, participants will self-administer cannabis containing either 0 mg THC (placebo) or 25 mg THC (active) via an oral route of administration. Ninety minutes after cannabis administration, participants will self-administer two oral capsules containing either 0 mg pregnenolone or 250 mg pregnenolone for a total of either 0 mg, 250 mg, or 500mg pregnenolone. Assessments will include subjective drug effect instruments, a battery of cognitive and psychomotor performance tasks, and physiological measures. Sessions will be conducted at a target rate of once per week. Results from this study of pregnenolone could have far-reaching clinical implications: not only would results provide conceptual support for NAMs as treatments for cannabis intoxication but may posit pregnenolone itself as a novel pharmacotherapeutic that could reduce the burden of ineffective and potentially harmful medications currently used in the treatment of cannabis intoxication in emergency settings. If pregnenolone is shown to be effective, additional drug development can be done to determine the best formulation, dose, and route of administration for maximal clinical benefit. Should pregnenolone not reverse THC intoxication completely, development of analogs with greater efficacy can be explored.

Notice

Study information shown on this site is derived from ClinicalTrials.gov (a public registry operated by the National Institutes of Health). The listing of studies provided is not certain to be all studies for which you might be eligible. Furthermore, study eligibility requirements can be difficult to understand and may change over time, so it is wise to speak with your medical care provider and individual research study teams when making decisions related to participation.